2-D DIGE analysis of UV-C radiation-responsive proteins in globe artichoke leaves

Sara Falvo, Mariasole Di Carli, Angiola Desiderio, Eugenio Benvenuto, Andrea Moglia, Twan America, Sergio Lanteri, Alberto Acquadro

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Plants respond to ultraviolet stress inducing a self-defence through the regulation of specific gene family members. The UV acclimation is the result of biochemical and physiological processes, such as enhancement of the antioxidant enzymatic system and accumulation of UV-absorbing phenolic compounds (e.g. flavonoids). Globe artichoke is an attractive species for studying the protein network involved in UV stress response, being characterized by remarkable levels of inducible antioxidants. Proteomic tools can assist the evaluation of the expression patterns of UV-responsive proteins and we applied the difference in-gel electrophoresis (DIGE) technology for monitoring the globe artichoke proteome variation at four time points following an acute UV-C exposure. A total of 145 UV-C-modulated proteins were observed and 119 were identified by LC-MS/MS using a ∼144000 customized Compositae protein database, which included about 19000 globe artichoke unigenes. Proteins were Gene Ontology (GO) categorized, visualized on their pathways and their behaviour was discussed. A predicted protein interaction network was produced and highly connected hub-like proteins were highlighted. Most of the proteins differentially modulated were chloroplast located, involved in photosynthesis, sugar metabolisms, protein folding and abiotic stress. The identification of UV-C-responsive proteins may contribute to shed light on the molecular mechanisms underlying plant responses to UV stress. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Original languageEnglish
Pages (from-to)448 - 460
Number of pages13
JournalProteomics
Volume12
Issue number3
DOIs
Publication statusPublished - Feb 2012

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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