A biosensor for the detection of triazine- and phenylurea-type herbicides was constructed using isolated Photosystem II (PS II) complexes as a biosensing element. PSII isolated from the thermophilic cyanobacterium Synechococcus elongatus was immobilized on the surface of a screen-printed sensor composed of a graphite working electrode and Ag/AgCI reference electrode deposited on a polymeric substrate. The biosensor was mounted in a flow microcell with illumination. The principle of the detection was based on the fact that herbicides selectively block PSII electron transport activity in a concentration-dependent manner. Changes of the activity were registered amperometrically as the rate of photoreduction of an artificial electron acceptor. The setup resulted in a reusable herbicide biosensor with a good stability (half-life of 24 h) and limit of detection of approximately 10-9M for diuron, atrazine and simazine. © 2002 Wiley Periodicals, Inc.
All Science Journal Classification (ASJC) codes
- Applied Microbiology and Biotechnology
Koblížek, M., Malý, J., Masojídek, J., Komenda, J., Kučera, T., Giardi, M. T., ... Pilloton, R. (2002). A biosensor for the detection of triazine and phenylurea herbicides designed using Photosystem II coupled to a screen-printed electrode. Biotechnology and Bioengineering, 78(1), 110 - 116. https://doi.org/10.1002/bit.10190