A biosensor for the detection of triazine and phenylurea herbicides designed using Photosystem II coupled to a screen-printed electrode

Michal Koblížek, Jan Malý, Jiří Masojídek, Josef Komenda, Tomáš Kučera, Maria T. Giardi, Autar K. Mattoo, Roberto Pilloton

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Abstract

A biosensor for the detection of triazine- and phenylurea-type herbicides was constructed using isolated Photosystem II (PS II) complexes as a biosensing element. PSII isolated from the thermophilic cyanobacterium Synechococcus elongatus was immobilized on the surface of a screen-printed sensor composed of a graphite working electrode and Ag/AgCI reference electrode deposited on a polymeric substrate. The biosensor was mounted in a flow microcell with illumination. The principle of the detection was based on the fact that herbicides selectively block PSII electron transport activity in a concentration-dependent manner. Changes of the activity were registered amperometrically as the rate of photoreduction of an artificial electron acceptor. The setup resulted in a reusable herbicide biosensor with a good stability (half-life of 24 h) and limit of detection of approximately 10-9M for diuron, atrazine and simazine. © 2002 Wiley Periodicals, Inc.
Original languageEnglish
Pages (from-to)110 - 116
Number of pages7
JournalBiotechnology and Bioengineering
Volume78
Issue number1
DOIs
Publication statusPublished - 5 Apr 2002
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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