A multi-biomarker analysis of the antioxidant efficacy of Parkinson's disease therapy

Monica Colamartino, Guglielmo Duranti, Roberta Ceci, Stefania Sabatini, Antonella Testa, Renata Cozzi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Substantial evidences suggest that reactive oxygen species participate in the normal aging process and in cancer and neurodegenerative age-related diseases. Parkinson's disease (PD), one of the most common oxidative stress-associated pathology in aging people, is treated with a standard pharmacological protocol consisting in a combined therapy L-dopa plus an inhibitor of dopa-decarboxylase, such as carbidopa. The therapy is well validated for the ability to restoring dopaminergic neurotransmission in PD patients, while L-dopa and carbidopa ability in modulating oxidative stress is currently under discussion. Our aim was to evaluate the impact of L-dopa and carbidopa on several biomarkers of exogenously-induced oxidative stress to validate the overall antioxidant effectiveness of the therapy. For this purpose we used peripheral blood lymphocytes from healthy donors treated in vitro with L-dopa and carbidopa and then challenged by different concentrations of H2O2. Glutathione (GSH, GSSG, GSH/GSSG), malondialdehyde (TBARs), protein carbonyls as well as DNA damage (8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) and micronuclei (MN)), modulation was evaluated. Our results show that L-dopa, but not carbidopa, decreases the markers of lipid and protein oxidation and increases the total content of glutathione. Both L-dopa and carbidopa (alone or in combination) are able to counteract the formation of 8-oxodG and to reduce H2O2-induced micronuclei.
Original languageEnglish
Pages (from-to)1 - 7
Number of pages7
JournalToxicology in Vitro
Volume47
DOIs
Publication statusPublished - 1 Mar 2018
Externally publishedYes

Fingerprint

Carbidopa
Levodopa
Biomarkers
Parkinson Disease
Antioxidants
Oxidative stress
Oxidative Stress
Glutathione Disulfide
Glutathione
Therapeutics
Aging of materials
Dopa Decarboxylase
Lymphocytes
Thiobarbituric Acid Reactive Substances
Pathology
Malondialdehyde
Synaptic Transmission
DNA Damage
Reactive Oxygen Species
Proteins

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

Colamartino, M., Duranti, G., Ceci, R., Sabatini, S., Testa, A., & Cozzi, R. (2018). A multi-biomarker analysis of the antioxidant efficacy of Parkinson's disease therapy. Toxicology in Vitro, 47, 1 - 7. https://doi.org/10.1016/j.tiv.2017.10.020
Colamartino, Monica ; Duranti, Guglielmo ; Ceci, Roberta ; Sabatini, Stefania ; Testa, Antonella ; Cozzi, Renata. / A multi-biomarker analysis of the antioxidant efficacy of Parkinson's disease therapy. In: Toxicology in Vitro. 2018 ; Vol. 47. pp. 1 - 7.
@article{a760a9125a7c4cb79b6eb8a61eb64438,
title = "A multi-biomarker analysis of the antioxidant efficacy of Parkinson's disease therapy",
abstract = "Substantial evidences suggest that reactive oxygen species participate in the normal aging process and in cancer and neurodegenerative age-related diseases. Parkinson's disease (PD), one of the most common oxidative stress-associated pathology in aging people, is treated with a standard pharmacological protocol consisting in a combined therapy L-dopa plus an inhibitor of dopa-decarboxylase, such as carbidopa. The therapy is well validated for the ability to restoring dopaminergic neurotransmission in PD patients, while L-dopa and carbidopa ability in modulating oxidative stress is currently under discussion. Our aim was to evaluate the impact of L-dopa and carbidopa on several biomarkers of exogenously-induced oxidative stress to validate the overall antioxidant effectiveness of the therapy. For this purpose we used peripheral blood lymphocytes from healthy donors treated in vitro with L-dopa and carbidopa and then challenged by different concentrations of H2O2. Glutathione (GSH, GSSG, GSH/GSSG), malondialdehyde (TBARs), protein carbonyls as well as DNA damage (8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) and micronuclei (MN)), modulation was evaluated. Our results show that L-dopa, but not carbidopa, decreases the markers of lipid and protein oxidation and increases the total content of glutathione. Both L-dopa and carbidopa (alone or in combination) are able to counteract the formation of 8-oxodG and to reduce H2O2-induced micronuclei.",
author = "Monica Colamartino and Guglielmo Duranti and Roberta Ceci and Stefania Sabatini and Antonella Testa and Renata Cozzi",
year = "2018",
month = "3",
day = "1",
doi = "10.1016/j.tiv.2017.10.020",
language = "English",
volume = "47",
pages = "1 -- 7",
journal = "Toxicology in Vitro",
issn = "0887-2333",
publisher = "Elsevier Limited",

}

Colamartino, M, Duranti, G, Ceci, R, Sabatini, S, Testa, A & Cozzi, R 2018, 'A multi-biomarker analysis of the antioxidant efficacy of Parkinson's disease therapy', Toxicology in Vitro, vol. 47, pp. 1 - 7. https://doi.org/10.1016/j.tiv.2017.10.020

A multi-biomarker analysis of the antioxidant efficacy of Parkinson's disease therapy. / Colamartino, Monica; Duranti, Guglielmo; Ceci, Roberta; Sabatini, Stefania; Testa, Antonella; Cozzi, Renata.

In: Toxicology in Vitro, Vol. 47, 01.03.2018, p. 1 - 7.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A multi-biomarker analysis of the antioxidant efficacy of Parkinson's disease therapy

AU - Colamartino, Monica

AU - Duranti, Guglielmo

AU - Ceci, Roberta

AU - Sabatini, Stefania

AU - Testa, Antonella

AU - Cozzi, Renata

PY - 2018/3/1

Y1 - 2018/3/1

N2 - Substantial evidences suggest that reactive oxygen species participate in the normal aging process and in cancer and neurodegenerative age-related diseases. Parkinson's disease (PD), one of the most common oxidative stress-associated pathology in aging people, is treated with a standard pharmacological protocol consisting in a combined therapy L-dopa plus an inhibitor of dopa-decarboxylase, such as carbidopa. The therapy is well validated for the ability to restoring dopaminergic neurotransmission in PD patients, while L-dopa and carbidopa ability in modulating oxidative stress is currently under discussion. Our aim was to evaluate the impact of L-dopa and carbidopa on several biomarkers of exogenously-induced oxidative stress to validate the overall antioxidant effectiveness of the therapy. For this purpose we used peripheral blood lymphocytes from healthy donors treated in vitro with L-dopa and carbidopa and then challenged by different concentrations of H2O2. Glutathione (GSH, GSSG, GSH/GSSG), malondialdehyde (TBARs), protein carbonyls as well as DNA damage (8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) and micronuclei (MN)), modulation was evaluated. Our results show that L-dopa, but not carbidopa, decreases the markers of lipid and protein oxidation and increases the total content of glutathione. Both L-dopa and carbidopa (alone or in combination) are able to counteract the formation of 8-oxodG and to reduce H2O2-induced micronuclei.

AB - Substantial evidences suggest that reactive oxygen species participate in the normal aging process and in cancer and neurodegenerative age-related diseases. Parkinson's disease (PD), one of the most common oxidative stress-associated pathology in aging people, is treated with a standard pharmacological protocol consisting in a combined therapy L-dopa plus an inhibitor of dopa-decarboxylase, such as carbidopa. The therapy is well validated for the ability to restoring dopaminergic neurotransmission in PD patients, while L-dopa and carbidopa ability in modulating oxidative stress is currently under discussion. Our aim was to evaluate the impact of L-dopa and carbidopa on several biomarkers of exogenously-induced oxidative stress to validate the overall antioxidant effectiveness of the therapy. For this purpose we used peripheral blood lymphocytes from healthy donors treated in vitro with L-dopa and carbidopa and then challenged by different concentrations of H2O2. Glutathione (GSH, GSSG, GSH/GSSG), malondialdehyde (TBARs), protein carbonyls as well as DNA damage (8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) and micronuclei (MN)), modulation was evaluated. Our results show that L-dopa, but not carbidopa, decreases the markers of lipid and protein oxidation and increases the total content of glutathione. Both L-dopa and carbidopa (alone or in combination) are able to counteract the formation of 8-oxodG and to reduce H2O2-induced micronuclei.

UR - http://www.scopus.com/inward/record.url?scp=85032790298&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85032790298&partnerID=8YFLogxK

U2 - 10.1016/j.tiv.2017.10.020

DO - 10.1016/j.tiv.2017.10.020

M3 - Article

VL - 47

SP - 1

EP - 7

JO - Toxicology in Vitro

JF - Toxicology in Vitro

SN - 0887-2333

ER -