A multi-biomarker analysis of the antioxidant efficacy of Parkinson's disease therapy

Monica Colamartino, Guglielmo Duranti, Roberta Ceci, Stefania Sabatini, Antonella Testa, Renata Cozzi

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Abstract

Substantial evidences suggest that reactive oxygen species participate in the normal aging process and in cancer and neurodegenerative age-related diseases. Parkinson's disease (PD), one of the most common oxidative stress-associated pathology in aging people, is treated with a standard pharmacological protocol consisting in a combined therapy L-dopa plus an inhibitor of dopa-decarboxylase, such as carbidopa. The therapy is well validated for the ability to restoring dopaminergic neurotransmission in PD patients, while L-dopa and carbidopa ability in modulating oxidative stress is currently under discussion. Our aim was to evaluate the impact of L-dopa and carbidopa on several biomarkers of exogenously-induced oxidative stress to validate the overall antioxidant effectiveness of the therapy. For this purpose we used peripheral blood lymphocytes from healthy donors treated in vitro with L-dopa and carbidopa and then challenged by different concentrations of H2O2. Glutathione (GSH, GSSG, GSH/GSSG), malondialdehyde (TBARs), protein carbonyls as well as DNA damage (8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) and micronuclei (MN)), modulation was evaluated. Our results show that L-dopa, but not carbidopa, decreases the markers of lipid and protein oxidation and increases the total content of glutathione. Both L-dopa and carbidopa (alone or in combination) are able to counteract the formation of 8-oxodG and to reduce H2O2-induced micronuclei.
Original languageEnglish
Pages (from-to)1 - 7
Number of pages7
JournalToxicology in Vitro
Volume47
DOIs
Publication statusPublished - 1 Mar 2018
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

Colamartino, M., Duranti, G., Ceci, R., Sabatini, S., Testa, A., & Cozzi, R. (2018). A multi-biomarker analysis of the antioxidant efficacy of Parkinson's disease therapy. Toxicology in Vitro, 47, 1 - 7. https://doi.org/10.1016/j.tiv.2017.10.020