A bacterial strain (FPA1) capable of using terbuthylazine, simazine, atrazine, 2-hydroxysimazine, deethylatrazine, isopropylamine or ethylamine as its sole carbon source was isolated from a shallow aquifer chronically contaminated with s-triazine herbicides. Based on its 16S rDNA sequence analysis, the strain FPA1 was identified as Rhodococcus wratislaviensis. The disappearance time of 50% of the initial terbuthylazine concentration in the presence of this strain (DT50) was 62 days. This strain was also able to mineralise the [U-ring14C] triazine-ring, albeit at a slow rate. A 16S rRNA target oligonucleotide probe (RhLu) was designed, and the FISH protocol was optimised, in order to detect R. wratislaviensis in s-triazine-contaminated sites. The RhLu probe gave a positive signal (expressed as % of total DAPI-positive cells) in both the groundwater (2.19 ± 0.41%) and soil (2.10 ± 0.96%) samples analysed. Using the RhLu probe, R. wratislaviensis can be readily detected, and its population dynamics can be easily monitored, in soil and in water ecosystems contaminated with s-triazine. To the best of our knowledge, this is the first report showing the isolation, from groundwater, of a bacterial strain able to degrade s-triazines. © 2009 Elsevier Ltd. All rights reserved.
All Science Journal Classification (ASJC) codes
- Ecological Modelling
- Water Science and Technology
- Waste Management and Disposal
Grenni, P., Gibello, A., Barra Caracciolo, A., Fajardo, C., Nande, M., Vargas, R., ... Martín, M. (2009). A new fluorescent oligonucleotide probe for in situ detection of s-triazine-degrading Rhodococcus wratislaviensis in contaminated groundwater and soil samples. Water Research, 43(12), 2999 - 3008. https://doi.org/10.1016/j.watres.2009.04.022