A sensitive photosystem II-based biosensor for detection of a class of herbicides

Michal Koblizek, Jiri Masojidek, Josef Komenda, Tomas Kucera, Roberte Pilloton, Autar K. Mattoo, Maria T. Giardi

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Abstract

We have developed a biosensor for the detection of residual triazine-, urea- and phenolic-type herbicides, using isolated photosystem II (PSII) particles from the thermophilic cyanobacterium, Synechococcus elongatus, as biosensing elements. The herbicide detection was based on the fact that, in the presence of artificial electron acceptors, the light-induced electron transfer through isolated PSII particles is accompanied by the release of oxygen, which is inhibited by the herbicide in a concentration-dependent manner. The PSII particles were immobilized between dialysis membrane and the Teflon membrane of the Clark oxygen electrode mounted in a flow cell that was illuminated. Inclusion of the antibiotic chloramphenicol in the reaction mixtures prolonged, by 50%, the lifetime of the biosensor. The use of highly active PSII particles in combination with the flow system resulted in a reusable herbicide biosensor with good stability (50% of initial activity was still remaining after 35-h use at 25°C) and high sensitivity (detection limit for diuron was 5 x 10-10M).
Original languageEnglish
Pages (from-to)664 - 669
Number of pages6
JournalBiotechnology and Bioengineering
Volume60
Issue number6
DOIs
Publication statusPublished - 20 Dec 1998
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Koblizek, M., Masojidek, J., Komenda, J., Kucera, T., Pilloton, R., Mattoo, A. K., & Giardi, M. T. (1998). A sensitive photosystem II-based biosensor for detection of a class of herbicides. Biotechnology and Bioengineering, 60(6), 664 - 669. https://doi.org/10.1002/(SICI)1097-0290(19981220)60:6<664::AID-BIT3>3.0.CO;2-B