To assess strawberry (Fragaria x ananassa Duch.) fruit quality and correlate gene expression data with those from biochemical analyses, we analyzed 3161 expressed sequence tags (ESTs) amplified from a fruit cDNA library. The 25 largest contigs related to plant genes with assigned functions comprised 499 ESTs (16% of the total), including genes not only associated with ripening (cell wall metabolism, sugar and acid synthesis, pigment formation, vitamin synthesis and allergenic properties), but also with plant-pathogen interactions, abiotic stresses and housekeeping functions. Over 1800 representative ESTs were used to produce cDNA micro-arrays to carry out comparative profiling experiments with genotypes of different fruit quality. Such microarray experiments have shown a number of genes whose differential expression correlated with differences among genotypes in important quality traits such as fruit firmness (cinnamoyl-CoA reductase, cinnamyl alcohol dehydrogenase, expansin, endo-β-1,4-glucanase) and volatile compound composition (alcohol acyl transferase).
|Publication status||Published - 2004|
All Science Journal Classification (ASJC) codes
Rosati, C., Mourgues, F., Giorno, F., Carbone, F., Carboni, M. A., & Perrotta, G. (2004). A strawberry EST database for evaluating fruit quality traits and selecting improved genotypes through cDNA microarrays. https://doi.org/10.17660/ActaHortic.2004.663.46