Activation of p53-dependent responses in tumor cells treated with a PARC-interacting peptide

Roberta Vitali, Vincenzo Cesi, Barbara Tanno, Giovanna Ferrari-Amorotti, Carlo Dominici, Bruno Calabretta, Giuseppe Raschellà

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We tested the activity of a p53 carboxy-terminal peptide containing the PARC-interacting region in cancer cells with wild type cytoplasmic p53. Peptide delivery was achieved by fusing it to the TAT transduction domain (TAT-p53-C-ter peptide). In a two-hybrid assay, the tetramerization domain (TD) of p53 was necessary and sufficient to bind PARC. The TAT-p53-C-ter peptide disrupted the PARC-p53 complex. Peptide treatment caused p53 nuclear relocation, p53-dependent changes in gene expression and enhancement of etoposide-induced apoptosis. These studies suggest that PARC-interacting peptides are promising candidates for the enhancement of p53-dependent apoptosis in tumors with wt cytoplasmic p53. © 2008 Elsevier Inc. All rights reserved.
Original languageEnglish
Pages (from-to)350 - 356
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume368
Issue number2
DOIs
Publication statusPublished - 4 Apr 2008
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

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