Adverse effects of cadmium exposure on mouse sperm

Helena Oliveira, Marcello Spanò, Conceição Santos, Maria de Lourdes Pereira

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The effects of cadmium chloride exposure on sperm functional parameters were evaluated on eight-week-old ICR-CD1 male mice administered with a single s.c. injection of 1, 2 and 3 mg CdCl2/kg bw. Groups of animals treated with each dose, as well as their respective controls, were sacrificed after 24 h to detect short-term (acute) effects and after 35 days. Sperm cells were collected from the epididymis and several parameters of sperm quality and function were evaluated, namely density, morphology, motility, viability, mitochondrial function, acrosome integrity, together with DNA fragmentation assessed by the TUNEL assay. The short-term effects of cadmium chloride resulted in an increased fraction of sperm with abnormal morphology, premature acrosome reaction and reduced motility. Late term effects (after 35 days) included a drastic reduction of sperm cell numbers and sperm motility. An increase in DNA fragmentation was also detected. © 2009 Elsevier Inc. All rights reserved.
Original languageEnglish
Pages (from-to)550 - 555
Number of pages6
JournalReproductive Toxicology
Issue number4
Publication statusPublished - Dec 2009
Externally publishedYes


All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

Oliveira, H., Spanò, M., Santos, C., & Pereira, M. D. L. (2009). Adverse effects of cadmium exposure on mouse sperm. Reproductive Toxicology, 28(4), 550 - 555.