Alkaline phosphatase inhibition based electrochemical sensors for the detection of pesticides

Franco Mazzei, Francesco Botrè, Simona Montilla, Roberto Pilloton, Elisabetta Podestà, Claudio Botrè

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Abstract

This article presents a bioelectrochemical system for the determination of pesticides by enzyme inhibition data. Measurements are performed by electrochemically monitoring the inhibition of the catalytic activity of the enzyme alkaline phosphatase (ALP) either in the presence or in the absence of pesticides, and particularly of Malathion and of 2,4-dichlorophenoxyacetic acid (2,4-D), representatives of the general classes of organophosphorous and organochlorinated agents. ALP catalytic activity was determined by using two different analytical configurations: (a) an amperometric ALP based biosensors using 3-indoxyl phosphate as the enzyme substrate; (b) a system allowing the voltammetric determination of the electroactive products of the ALP catalyzed reactions by using two different substrates: phenyl phosphate (PP) and ascorbate-2-phosphate (A-2-P). Studies of cyclic voltammetry and amperometry were performed first to define the optimal experimental conditions of the electrochemical measurements. For the optimal experimental conditions (Tris-0.1 M HC1 aqueous buffer solution at pH 8.0, incubation time of 30-60 min) a detection limit of 0.5-6 μg 1-1is obtained, with a response, which is linear over 1-2 decades of concentration. © 2004 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)95 - 100
Number of pages6
JournalJournal of Electroanalytical Chemistry
Volume574
Issue number1
DOIs
Publication statusPublished - 15 Dec 2004
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Electrochemistry

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