An antibody-based microarray assay for the simultaneous detection of aflatoxin B

Ilaria Lamberti, Caterina Tanzarella, Isabella Solinas, Cristiano Padula, Lucia Mosiello

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Advances in microsystem technology have enabled protein and nucleic acid-based microarrays to be used in various applications, including the study of diseases, drug discovery, genetic screening, and clinical and food diagnostics. Analytical methods for the detection of mycotoxins, however, remain largely based on thin layer chromatography (TLC), high pressure liquid chromatography (HPLC), or enzyme-linked Immunosorbent assay (ELISA) . The aim of our work, therefore, was to transfer an immunological assay from microtitrr plates into microarray format, in order to develop a multiparametric, rapid, sensitive and inexpensive method for the detection of mycotoxins for use in food safety applications. Microarray technology enables the fast and parallel analysis of a multitude of biologically relevant parameters. Not only nucleic acid-based tests but also peptide, antigen, and antibody assays, using different formats of microarrays, have evolved within the last decade. Antibody-based microarrays provide a powerful tool that can be used to generate rapid and detailed expression profiles of a defined set of analytes in complex samples and are potentially useful for generating rapid immunological assays of food contaminants. In this paper, we report a feasibility study of the application of antibody microarrays for the simultaneous (or independent) detection of two common mycotoxins, Aflatoxin B1and Fumonisin B1. We present the development of microarray detection of aflatoxin B1 and fumonisin B1 in standard solutions with detection limits of 3 ng/ml of AFB1 and 43 ng/ml for FB1, and have developed a competitive immunoassay in microarray format for simultaneous analyses. The quality of the microarray data is comparable to data generated by microplate-based immunoassay (ELISA), but further investigations are needed in order to characterise our method more fully. We hope that these preliminary results might suggest that further research is warranted in order to develop hapten microarrays for the immunochemical simultaneous analysis of mycotoxins, as well as for other small molecules (e.g. bacterial toxins or biological warfare agents). © 2009 Society for Mycotoxin Research and Springer.
Original languageEnglish
Pages (from-to)193 - 200
Number of pages8
JournalMycotoxin Research
Volume25
Issue number4
DOIs
Publication statusPublished - Dec 2009
Externally publishedYes

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Microbiology
  • Toxicology

Cite this

Lamberti, I., Tanzarella, C., Solinas, I., Padula, C., & Mosiello, L. (2009). An antibody-based microarray assay for the simultaneous detection of aflatoxin B. Mycotoxin Research, 25(4), 193 - 200. https://doi.org/10.1007/s12550-009-0028-9