Analysis of Radiation-Induced Chromosomal Aberrations on a Cell-by-Cell Basis after Alpha-Particle Microbeam Irradiation: Experimental Data and Simulations

Antonella Testa, Francesca Ballarini, Ulrich Giesen, Octávia Monteiro Gil, Mario P. Carante, John Tello, Frank Langner, Hans Rabus, Valentina Palma, Massimo Pinto, Clarice Patrono

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

There is a continued need for further clarification of various aspects of radiation-induced chromosomal aberration, including its correlation with radiation track structure. As part of the EMRP joint research project, Biologically Weighted Quantities in Radiotherapy (BioQuaRT), we performed experimental and theoretical analyses on chromosomal aberrations in Chinese hamster ovary cells (CHO-K1) exposed to α particles with final energies of 5.5 and 17.8 MeV (absorbed doses: ∼2.3 Gy and ∼1.9 Gy, respectively), which were generated by the microbeam at the Physikalisch-Technische Bundesanstalt (PTB) in Braunschweig, Germany. In line with the differences in linear energy transfer (approximately 85 keV/μm for 5.5 MeV and 36 keV/μm for 17.8 MeV α particles), the 5.5 MeV α particles were more effective than the 17.8 MeV α particles, both in terms of the percentage of aberrant cells (57% vs. 33%) and aberration frequency. The yield of total aberrations increased by a factor of ∼2, although the increase in dicentrics plus centric rings was less pronounced than in acentric fragments. The experimental data were compared with Monte Carlo simulations based on the BIophysical ANalysis of Cell death and chromosomal Aberrations model (BIANCA). This comparison allowed interpretation of the results in terms of critical DNA damage cluster lesions (CLs). More specifically, the higher aberration yields observed for the 5.5 MeV α particles were explained by taking into account that, although the nucleus was traversed by fewer particles (nominally, 11 vs. 25), each particle was much more effective (by a factor of ∼3) at inducing CLs. This led to an increased yield of CLs per cell (by a factor of ∼1.4), consistent with the increased yield of total aberrations observed in the experiments.
Original languageEnglish
Pages (from-to)597 - 604
Number of pages8
JournalRadiation Research
Volume189
Issue number6
DOIs
Publication statusPublished - 1 Jun 2018
Externally publishedYes

Fingerprint

Alpha Particles
microbeams
Chromosome Aberrations
alpha particles
aberration
Radiation
irradiation
radiation
cells
Linear Energy Transfer
lesions
CHO Cells
simulation
Cricetulus
DNA Damage
Germany
Ovary
Cell Death
Radiotherapy
ovaries

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Radiation
  • Radiology Nuclear Medicine and imaging

Cite this

Testa, Antonella ; Ballarini, Francesca ; Giesen, Ulrich ; Gil, Octávia Monteiro ; Carante, Mario P. ; Tello, John ; Langner, Frank ; Rabus, Hans ; Palma, Valentina ; Pinto, Massimo ; Patrono, Clarice. / Analysis of Radiation-Induced Chromosomal Aberrations on a Cell-by-Cell Basis after Alpha-Particle Microbeam Irradiation: Experimental Data and Simulations. In: Radiation Research. 2018 ; Vol. 189, No. 6. pp. 597 - 604.
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Analysis of Radiation-Induced Chromosomal Aberrations on a Cell-by-Cell Basis after Alpha-Particle Microbeam Irradiation: Experimental Data and Simulations. / Testa, Antonella; Ballarini, Francesca; Giesen, Ulrich; Gil, Octávia Monteiro; Carante, Mario P.; Tello, John; Langner, Frank; Rabus, Hans; Palma, Valentina; Pinto, Massimo; Patrono, Clarice.

In: Radiation Research, Vol. 189, No. 6, 01.06.2018, p. 597 - 604.

Research output: Contribution to journalArticle

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T1 - Analysis of Radiation-Induced Chromosomal Aberrations on a Cell-by-Cell Basis after Alpha-Particle Microbeam Irradiation: Experimental Data and Simulations

AU - Testa, Antonella

AU - Ballarini, Francesca

AU - Giesen, Ulrich

AU - Gil, Octávia Monteiro

AU - Carante, Mario P.

AU - Tello, John

AU - Langner, Frank

AU - Rabus, Hans

AU - Palma, Valentina

AU - Pinto, Massimo

AU - Patrono, Clarice

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