Ion Torrent is a next generation sequencing technology based on the detection of hydrogen ions produced during DNA chain elongation; this technology allows analyzing and characterizing genomes, genes, and species. Here, we describe an Ion Torrent procedure applied to the metagenomic analysis of 16S rRNA gene amplicons to study the bacterial diversity in food and environmental samples.
All Science Journal Classification (ASJC) codes
- Molecular Biology
Fantini, E., Gianese, G., Giuliano, G., & Fiore, A. (2015). Bacterial metabarcoding by 16S rRNA gene ion torrent amplicon sequencing. Methods in Molecular Biology, 1231, 77 - 90. https://doi.org/10.1007/978-1-4939-1720-4_5