Bacterial metabarcoding by 16S rRNA gene ion torrent amplicon sequencing

Elio Fantini, Giulio Gianese, Giovanni Giuliano, Alessia Fiore

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7 Citations (Scopus)

Abstract

Ion Torrent is a next generation sequencing technology based on the detection of hydrogen ions produced during DNA chain elongation; this technology allows analyzing and characterizing genomes, genes, and species. Here, we describe an Ion Torrent procedure applied to the metagenomic analysis of 16S rRNA gene amplicons to study the bacterial diversity in food and environmental samples.
Original languageEnglish
Pages (from-to)77 - 90
Number of pages14
JournalMethods in Molecular Biology
Volume1231
DOIs
Publication statusPublished - 2015

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All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Medicine(all)

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