The insulin-like growth factor (IGF) axis is frequently activated in neuroblastoma (NB) tumors and cell lines. We show that silencing endogenous expression of IGF Binding Protein-5 (IGFBP-5) in NB cells by using microRNA and siRNA causes mitochondrial apoptosis that is characterized by: (a) release of cytochrome C in the cytoplasm and activation of caspase 9; (b) Erk1 and Erk2 inhibition; and (c) upregulation of pro-apoptotic proteins Bim and Bax. Bim upregulation is caused, at least in part, by protein stabilization that may depend on inhibition of Erk1 and Erk2. Of interest, Bim knock-down by siRNA decreases apoptosis in IGFBP-5-interfered cells. Thus, inhibition of endogenously produced IGFBP-5 is associated with Bim-dependent apoptosis in NB cells. © 2006 Elsevier Inc. All rights reserved.
|Pages (from-to)||547 - 552|
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - 15 Dec 2006|
All Science Journal Classification (ASJC) codes
- Molecular Biology
Tanno, B., Vitali, R., Arcangelis, D. D., Mancini, C., Eleuteri, P., Dominici, C., & Raschellà, G. (2006). Bim-dependent apoptosis follows IGFBP-5 down-regulation in neuroblastoma cells. Biochemical and Biophysical Research Communications, 351(2), 547 - 552. https://doi.org/10.1016/j.bbrc.2006.10.062