Calibration curves for biological dosimetry by fluorescence in situ hybridisation

L. Stronati, M. Durante, G. Gensabella, G. Gialanella, G.F. Grossi, M. Pugliese, P. Scampoli, A. Sgura, A. Testa, C. Tanzarella

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Abstract

Dose-response curves were measured for the induction of chromosomal aberrations in peripheral blood lymphocytes after acute exposure in vitro to60Co γ rays. Blood was obtained from four different healthy donors, and chromosomes were either observed at metaphase, following colcemid accumulation, or prematurely condensed by calyculin A. Cells were analysed in three different Italian laboratories. Chromosomes 1, 2, and 4 were painted, and simple-type interchanges between painted and non-painted chromosomes were scored in cells exposed in the dose range 0.1-3.0 Gy. The chemical-induced premature chromosome condensation method was also used combined with chromosome painting (chromosome 4 only) to determine calibration curves for high dose exposures (up to 20 Gy X rays). Calibration curves described in this paper will be used in our laboratories for biological dosimetry by fluorescence in situ hybridisation.
Original languageEnglish
Pages (from-to)335 - 345
Number of pages11
JournalRadiation Protection Dosimetry
Volume94
Issue number4
Publication statusPublished - 2001
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Radiological and Ultrasound Technology
  • Radiation
  • Radiology Nuclear Medicine and imaging
  • Public Health, Environmental and Occupational Health

Cite this

Stronati, L., Durante, M., Gensabella, G., Gialanella, G., Grossi, G. F., Pugliese, M., ... Tanzarella, C. (2001). Calibration curves for biological dosimetry by fluorescence in situ hybridisation. Radiation Protection Dosimetry, 94(4), 335 - 345.