Two clones derived from neuroblastoma cell line LAN-5 transfected with an expression vector carrying a domain of the c-myb oncogene in antisense (AS) orientation, were characterized for their proliferative and differentiative behaviour. AS clones transcribed an antisense RNA which can prevent the translation of the c-myb message by forming a RNA-RNA hybrid. While they retained the ability to differentiate under retinoic acid treatment they showed a marked reduction in proliferation and a stronger sensitivity to serum starvation conditions as compared to controls. These findings suggest a role of c-myb in proliferative processes in neuroblastoma cells.
|Pages (from-to)||245 - 251|
|Number of pages||7|
|Journal||Clinical Chemistry and Enzymology Communications|
|Publication status||Published - 1993|
All Science Journal Classification (ASJC) codes
- Clinical Biochemistry
Raschella, G., Pucci, S., Negroni, A., Romeo, A., Margutti, P., & Calabretta, B. (1993). Characterization of neuroblastoma cell clones transfected with an expression vector transcribing antisense RNA to c-myb. Clinical Chemistry and Enzymology Communications, 5(4-6), 245 - 251.