Characterization of neuroblastoma cell clones transfected with an expression vector transcribing antisense RNA to c-myb

G. Raschella, S. Pucci, A. Negroni, A. Romeo, P. Margutti, B. Calabretta

Research output: Contribution to journalArticle

Abstract

Two clones derived from neuroblastoma cell line LAN-5 transfected with an expression vector carrying a domain of the c-myb oncogene in antisense (AS) orientation, were characterized for their proliferative and differentiative behaviour. AS clones transcribed an antisense RNA which can prevent the translation of the c-myb message by forming a RNA-RNA hybrid. While they retained the ability to differentiate under retinoic acid treatment they showed a marked reduction in proliferation and a stronger sensitivity to serum starvation conditions as compared to controls. These findings suggest a role of c-myb in proliferative processes in neuroblastoma cells.
Original languageEnglish
Pages (from-to)245 - 251
Number of pages7
JournalClinical Chemistry and Enzymology Communications
Volume5
Issue number4-6
Publication statusPublished - 1993
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

Cite this

Raschella, G., Pucci, S., Negroni, A., Romeo, A., Margutti, P., & Calabretta, B. (1993). Characterization of neuroblastoma cell clones transfected with an expression vector transcribing antisense RNA to c-myb. Clinical Chemistry and Enzymology Communications, 5(4-6), 245 - 251.