Chemical Profile and Antioxidant Properties of Extracts and Essential Oils from Citrus × limon (L.) Burm. cv. Femminello Comune

Monica Rosa Loizzo, Rosa Tundis, Marco Bonesi, Giuseppe Di Sanzo, Alessandra Verardi, Catia Giovanna Lopresto, Alessandro Pugliese, Francesco Menichini, Roberto Balducchi, Vincenza Calabrò

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Abstract

Citrus × limon cv. Femminello Comune (Rutaceae) from Rocca Imperiale (Italy), one of the six Protected Geographical Indication (PGI) Italian lemon crops, has been recently received renewed interest. In this work, fresh and dried peels and leaves were extracted by hydrodistillation, supercritical fluid extraction (SFE), and Soxhlet apparatus. Chemical profile was assessed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). Except for leaves extracts obtained by Soxhlet apparatus, the monoterpene hydrocarbons fraction dominated. Limonene, γ-terpinene, and β-pinene were the main identified compounds. The antioxidant activity was investigated using different in vitro assays namely 2,2-diphenyl-1-picrylhydrazyl (DPPH), ABTS, ferric reducing ability power (FRAP), and β-carotene bleaching test. In DPPH test, the essential oil obtained by hydrodistillation of fresh peel exhibited the highest activity (IC50 of 1.17 mg/ml). Leaves extracted by SFE showed a good activity in both DPPH and β-carotene bleaching test with IC50 values of 2.20 and 6.66 mg/ml, respectively. Monoterpene hydrocarbons fraction exhibited a positive Pearson's correlation coefficient with all antioxidant assays. Leaves, often considered waste material, should be considered from a different point because they represent a matrix of indisputable interest.
Original languageEnglish
Pages (from-to)571 - 581
Number of pages11
JournalChemistry and Biodiversity
Volume13
Issue number5
DOIs
Publication statusPublished - 1 May 2016
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemistry(all)
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology

Cite this

Loizzo, M. R., Tundis, R., Bonesi, M., Sanzo, G. D., Verardi, A., Lopresto, C. G., ... Calabrò, V. (2016). Chemical Profile and Antioxidant Properties of Extracts and Essential Oils from Citrus × limon (L.) Burm. cv. Femminello Comune. Chemistry and Biodiversity, 13(5), 571 - 581. https://doi.org/10.1002/cbdv.201500186