Classification of actinoplanes sp. ATCC 33076, an actinomycete that produces the glycolipodepsipeptide antibiotic ramoplanin, as actinoplanes ramoplaninifer sp. Nov

Giorgia Letizia Marcone, Elisa Binda, Marcella Reguzzoni, Luciano Gastaldo, Claudia Dalmastri, Flavia Marinelli

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Abstract

Strain ATCC 33076, which produces the antibiotic ramoplanin, was isolated from a soil sample collected in India, and it was classified as a member of the genus Actinoplanes on the basis of morphology and cell-wall composition. A phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain forms a distinct clade within the genus Actinoplanes, and it is most closely related to Actinoplanes deccanensis IFO 13994T(98.71% similarity) and Actinoplanes atraurantiacus Y16T(98.33 %). The strain forms an extensively branched substrate mycelium; the sporangia are formed very scantily and are globose with irregular surface. Spores are oval and motile. The cell wall contains meso-diaminopimelic acid and the diagnostic sugars are xylose and arabinose. The predominant menaquinone is MK-9(H6), with minor amounts of MK-9(H4) and MK-9(H2). Mycolic acids are absent. The diagnostic phospholipids are phosphatidylethanolamine, hydroxyphosphatidylethanolamine and phosphatidylglycerol. The major cellular fatty acids are anteiso-C17: 0and iso-C16: 0, followed by iso-C15: 0and moderate amounts of anteiso-C15: 0, iso- C17: 0and C18: 1ω9c. The genomic DNA G+C content is 71.4 mol%. Significant differences in the morphological, chemotaxonomic and biochemical data, together with DNA–DNA relatedness between strain ATCC 33076 and closely related type strains, clearly demonstrated that strain ATCC 33076 represents a novel species of the genus Actinoplanes, for which the name Actinoplanes ramoplaninifer sp. Nov. is proposed. The type strain is ATCC 33076T(=DSM 105064T=NRRL B-65484T).
Original languageEnglish
Article number002281
Pages (from-to)4181 - 4188
Number of pages8
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume67
Issue number10
DOIs
Publication statusPublished - 1 Oct 2017
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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