Strain ATCC 33076, which produces the antibiotic ramoplanin, was isolated from a soil sample collected in India, and it was classified as a member of the genus Actinoplanes on the basis of morphology and cell-wall composition. A phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain forms a distinct clade within the genus Actinoplanes, and it is most closely related to Actinoplanes deccanensis IFO 13994T(98.71% similarity) and Actinoplanes atraurantiacus Y16T(98.33 %). The strain forms an extensively branched substrate mycelium; the sporangia are formed very scantily and are globose with irregular surface. Spores are oval and motile. The cell wall contains meso-diaminopimelic acid and the diagnostic sugars are xylose and arabinose. The predominant menaquinone is MK-9(H6), with minor amounts of MK-9(H4) and MK-9(H2). Mycolic acids are absent. The diagnostic phospholipids are phosphatidylethanolamine, hydroxyphosphatidylethanolamine and phosphatidylglycerol. The major cellular fatty acids are anteiso-C17: 0and iso-C16: 0, followed by iso-C15: 0and moderate amounts of anteiso-C15: 0, iso- C17: 0and C18: 1ω9c. The genomic DNA G+C content is 71.4 mol%. Significant differences in the morphological, chemotaxonomic and biochemical data, together with DNA–DNA relatedness between strain ATCC 33076 and closely related type strains, clearly demonstrated that strain ATCC 33076 represents a novel species of the genus Actinoplanes, for which the name Actinoplanes ramoplaninifer sp. Nov. is proposed. The type strain is ATCC 33076T(=DSM 105064T=NRRL B-65484T).
|Pages (from-to)||4181 - 4188|
|Number of pages||8|
|Journal||International Journal of Systematic and Evolutionary Microbiology|
|Publication status||Published - 1 Oct 2017|
All Science Journal Classification (ASJC) codes
- Ecology, Evolution, Behavior and Systematics
Marcone, G. L., Binda, E., Reguzzoni, M., Gastaldo, L., Dalmastri, C., & Marinelli, F. (2017). Classification of actinoplanes sp. ATCC 33076, an actinomycete that produces the glycolipodepsipeptide antibiotic ramoplanin, as actinoplanes ramoplaninifer sp. Nov. International Journal of Systematic and Evolutionary Microbiology, 67(10), 4181 - 4188. . https://doi.org/10.1099/ijsem.0.002281