Comparison of 7,12-Dimethylbenz(a)anthracene-DNA Adduction in the Epidermis of Two Lines of Mice Selected for Resistance (CAR-R) or Susceptibility (CAR-S) to Skin Carcinogenesis

François Perin, Odette Perin, Nicole Barat, Marie Jose Plessis, Anna Saran, Claudio Pioli, Vincenzo Covelli, Guido Biozzi, Denise Mouton

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Two lines of mice were produced by bidirectional selective breeding: one resistant (CAR-R) and one susceptible (CAR-S) to two-stage skin carcinogenesis by dimethylbenz(a)anthracene and 12-O-tetradecanoylphorbol-13-acetate. The dimethylbenz(a)anthracene-DNA adduct formation was compared in the two lines by a postlabeling procedure so as to determine whether the striking interline difference observed as to tumor incidence could (in part) be due to differences in the formation of DNA-reactive metabolites. Results show that qualitatively, adduct profiles in CAR-R and CAR-S epidermis are similar. Quantitatively, the total binding level is slightly higher in CAR-S versus CAR-R mice during the 30-day follow-up. However, these minor differences do not increase in function of the response to selection observed through three consecutive generations. A 2- or 4-week promotion with 12-0-tetradecanoylphorbol-13-acetate enhances the decrease of adduct level in the two lines. This effect is somewhat more pronounced in CAR-S mice. Results strongly suggest that the expression of the genes responsible for CAR-R/CAR-S phenotypic difference affects mainly the postinitiation stages. © 1994, American Association for Cancer Research. All rights reserved.
Original languageEnglish
Pages (from-to)4635 - 4640
Number of pages6
JournalCancer Research
Issue number17
Publication statusPublished - 1994
Externally publishedYes


All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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