Two lines of mice were produced by bidirectional selective breeding: one resistant (CAR-R) and one susceptible (CAR-S) to two-stage skin carcinogenesis by dimethylbenz(a)anthracene and 12-O-tetradecanoylphorbol-13-acetate. The dimethylbenz(a)anthracene-DNA adduct formation was compared in the two lines by a postlabeling procedure so as to determine whether the striking interline difference observed as to tumor incidence could (in part) be due to differences in the formation of DNA-reactive metabolites. Results show that qualitatively, adduct profiles in CAR-R and CAR-S epidermis are similar. Quantitatively, the total binding level is slightly higher in CAR-S versus CAR-R mice during the 30-day follow-up. However, these minor differences do not increase in function of the response to selection observed through three consecutive generations. A 2- or 4-week promotion with 12-0-tetradecanoylphorbol-13-acetate enhances the decrease of adduct level in the two lines. This effect is somewhat more pronounced in CAR-S mice. Results strongly suggest that the expression of the genes responsible for CAR-R/CAR-S phenotypic difference affects mainly the postinitiation stages. © 1994, American Association for Cancer Research. All rights reserved.
|Pages (from-to)||4635 - 4640|
|Number of pages||6|
|Publication status||Published - 1994|
All Science Journal Classification (ASJC) codes
- Cancer Research
Perin, F., Perin, O., Barat, N., Plessis, M. J., Saran, A., Pioli, C., ... Mouton, D. (1994). Comparison of 7,12-Dimethylbenz(a)anthracene-DNA Adduction in the Epidermis of Two Lines of Mice Selected for Resistance (CAR-R) or Susceptibility (CAR-S) to Skin Carcinogenesis. Cancer Research, 54(17), 4635 - 4640.