Construction and comparison of trametes versicolor laccase biosensors capable of detecting xenobiotics

Mustafa Kemal Sezgintrk, Dilek Odaci, Nurdan Pazarliolu, Roberto Pilloton, Erhan Dinçkaya, Azmi Telefoncu, Suna Timur

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Abstract

Amperometric biosensors using laccase from Trametes versicolor as a bioelement were developed for 2,4-dichloro phenoxy acetic acid (2,4-D). Laccase enzyme was immobilized by gelatin and glutaraldehyde onto a Clark oxygen probe and screen printed electrodes (SPEs). Amperometric and chronoamperometric measurements were carried out with the biosensors. First, the effect of laccase activity on the biosensor performances was investigated for both biosensors, and then optimum pH and temperature and also thermal stability of the biosensors were tested. In addition, the detection ranges of some phenolic compounds were obtained by the help of calibration graphs of them. In repeatability studies, variation coefficients and standard deviations for both biosensors were also calculated by the studies done for this purposes. Finally, the biosensors were applied to the determination of 2,4-D in a real herbicide sample. © 2010 Informa UK Ltd.
Original languageEnglish
Pages (from-to)192 - 199
Number of pages8
JournalArtificial Cells, Nanomedicine and Biotechnology
Volume38
Issue number4
DOIs
Publication statusPublished - Aug 2010
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biomedical Engineering

Cite this

Sezgintrk, M. K., Odaci, D., Pazarliolu, N., Pilloton, R., Dinçkaya, E., Telefoncu, A., & Timur, S. (2010). Construction and comparison of trametes versicolor laccase biosensors capable of detecting xenobiotics. Artificial Cells, Nanomedicine and Biotechnology, 38(4), 192 - 199. https://doi.org/10.3109/10731191003776777