Determination of phenolic acids using Trametes versicolor laccase

Dilek Odaci, Suna Timur, Nurdan Pazarlioglu, Maria Rita Montereali, Walter Vastarella, Roberto Pilloton, Azmi Telefoncu

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Abstract

Two biosensors based on Trametes versicolor laccase (TvL) were developed for the determination of phenolic compounds. Commercial oxygen electrode and ferrocene-modified screen-printed graphite electrodes were used for preparation of laccase biosensors. The systems were calibrated for three phenolic acids. Linearity was obtained in the concentration range 0.1-1.0 μM caffeic acid, 0.05-0.2 μM ferulic acid, 2.0-14.0 μM syringic acid for laccase immobilised on a commercial oxygen electrode and 2.0-30.0 μM caffeic acid, 2.0-10.0 μM ferulic acid, 4.0-30.0 μM syringic acid for laccase immobilised on ferrocene-modified screen-printed electrodes. Furthermore, optimal pH, temperature and thermal stability studies were performed with the commercial oxygen electrode. Both electrodes were used for determination of a class of phenolic acids, achieving a cheap and fast tool and an easy to be used procedure for screening real samples of human plasma. © 2006 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)312 - 317
Number of pages6
JournalTalanta
Volume71
Issue number1
DOIs
Publication statusPublished - 15 Jan 2007
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Spectroscopy

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