DNA damage and related modifier genes in Italian cystic fibrosis patients

Silvia Sterpone, Tommaso Cornetta, Adriano Angioni, Ersilia Fiscarelli, Vincenzina Lucidi, Antonella Testa, Renata Cozzi

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5 Citations (Scopus)

Abstract

Cystic Fibrosis (CF) is an autosomal recessive multisystemic disorder showing a highly heterogeneous phenotype, even among siblings carrying identical CFTR mutations. Moreover, oxidative stress is of central importance in the pathogenesis of cystic fibrosis. The present study seeks to value the presence of oxidative damage in CF patients and the possible modifier effect of repair and glutathione-S-transferase genes. We analysed the presence of DNA damage in leukocytes of 63 CF patients at an Italian CF centre and 63 controls, through the alkaline Comet assay to detect DNA strand breaks. Furthermore, controls and 93 CF subjects were genotyped for 5 genes by RFLP-PCR (XRCC1,OGG1,GSTP1) and PCR assay (GSTM1, GSTT1). No difference in Comet assay values was observed comparing controls to CF patients, although CF subjects showed slightly higher mean values. The crude Odds-Ratio (OR) was higher than one for XRCC1 and GSTP1 genotypes and liver status and for XRCC1 and OGG1 genotypes and pancreatic insufficiency, but in all cases the p-values were not significant. In this case-control study, neither DNA damage nor gene polymorphisms seem to influence CF manifestation.
Original languageEnglish
Pages (from-to)477 - 486
Number of pages10
JournalBiological Research
Volume42
Issue number4
DOIs
Publication statusPublished - 2009
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Sterpone, S., Cornetta, T., Angioni, A., Fiscarelli, E., Lucidi, V., Testa, A., & Cozzi, R. (2009). DNA damage and related modifier genes in Italian cystic fibrosis patients. Biological Research, 42(4), 477 - 486. https://doi.org/10.4067/S0716-97602009000400009