DNA damage repair and genetic polymorphisms: Assessment of individual sensitivity and repair capacity

Tommaso Cornetta, Fabiola Festa, Antonella Testa, Renata Cozzi

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Purpose: To study the repair capacity after X-ray irradiation in human peripheral blood cells of healthy subjects, in relation to their genotypes. Methods and Materials: The peripheral blood of 50 healthy subjects was irradiated in vitro with 2 Gy of X rays and the induced DNA damage was measured by Comet assay immediately after irradiation. DNA repair was detected by analyzing the cells at defined time intervals after the exposure. Furthermore, all subjects were genotyped for XRCC1, OGG1, and XPC genes. Results: After X-ray irradiation, persons bearing XRCC1 homozygous variant (codon 399) genotype exhibited significantly lower Tail DNA values than those bearing wild-type and heterozygous genotypes. These results are also confirmed at 30 and 60 min after irradiation. Furthermore, XPC heterozygous subjects (variant codon 939) showed lower residual DNA damage 60 min after irradiation compared with wild-type and homozygous genotypes. Conclusion: The results of the present study show that polymorphisms in DNA repair genes could influence individual DNA repair capacity. © 2006 Elsevier Inc. All rights reserved.
Original languageEnglish
Pages (from-to)537 - 545
Number of pages9
JournalInternational Journal of Radiation Oncology Biology Physics
Volume66
Issue number2
DOIs
Publication statusPublished - 1 Oct 2006
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Radiation
  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

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