Effect of conditions of three dimensional clinostating on testicular cell machinery

Bianca Maria Uva, Felice Strollo, Franco Ricci, Martina Pastorino, Ian J. Mason, Maria Angela Masini

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Abstract

Our scope was to study the effects of on ground random positioning machine rotation on swine testicular cells in culture. Cells of the 2 n karyotype line, from trypsinized swine testes, were submitted to modeled microgravity (μ G) using a 3D RPM from 15 min to 24 h. The cultured cells were then fixed and submitted to immunohistochemistry using antibodies to steroid dehydrogenases, heat shock proteins and the sodium pump (Na+/ K+ATPase). The results revealed that, after 15 min at modeled μ G, all the cells showed damages at cytoskeletal level. The immunoreactions for 3 β HSD, 17 β HSD and Na+/ K+ATPase were almost abolished. After 24 h of treatment, the presence of the enzymes was restored, and small heat shock proteins were strongly immunostainable. On the other hand, in the 1 × G cultures, the expression of HSPs was very weak. We conclude that modeled μ G by random positioning of cells affects testicular cells in culture for a short time, while the normal activity of the cells is restored after 24 h. © 2006 Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)391 - 396
Number of pages6
JournalActa Astronautica
Volume60
Issue number4-7 SPEC. ISS.
DOIs
Publication statusPublished - Feb 2007
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Aerospace Engineering

Cite this

Uva, B. M., Strollo, F., Ricci, F., Pastorino, M., Mason, I. J., & Angela Masini, M. (2007). Effect of conditions of three dimensional clinostating on testicular cell machinery. Acta Astronautica, 60(4-7 SPEC. ISS.), 391 - 396. https://doi.org/10.1016/j.actaastro.2006.09.007