Effects of 50 Hz Electromagnetic Field Exposure on Apoptosis and Differentiation in a Neuroblastoma Cell Line

M.C. Pirozzoli, C. Marino, G.A. Lovisolo, C. Laconi, L. Mosiello, A. Negroni

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Abstract

Experiments were carried out to assess whether a magnetic field of 50 Hz and 1 mT can influence apoptosis and proliferation in the human neuroblastoma cell line LAN-5. TUNEL assays and poly-ADP ribose polymerase (PARP) expression analysis were performed to test apoptosis induction, and the WST-1 assay was used to calculate the proliferation index in a long term exposure. No alterations were found in cellular ability to undergo programmed cell death, but a small increase in the proliferation index was evidenced after 7 days of continuous exposure. Also, a slight and transient increase of B-myb oncogene expression was detected after 5 days of exposure. Combined exposures of cells to EMF and to chemical agents which interfere with proliferation, such as the differentiative agent retinoic acid and the apoptotic inducer camptothecin, showed an antagonistic effect of magnetic fields against the differentiation of the LAN-5 cells and a protective effect towards apoptosis. © 2003 Wiley-Liss, Inc.
Original languageEnglish
Pages (from-to)510 - 516
Number of pages7
JournalBioelectromagnetics
Volume24
Issue number7
DOIs
Publication statusPublished - Oct 2003
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)
  • Biophysics

Cite this

Pirozzoli, M. C., Marino, C., Lovisolo, G. A., Laconi, C., Mosiello, L., & Negroni, A. (2003). Effects of 50 Hz Electromagnetic Field Exposure on Apoptosis and Differentiation in a Neuroblastoma Cell Line. Bioelectromagnetics, 24(7), 510 - 516. https://doi.org/10.1002/bem.10130