Enrichment of activated sludge for enhanced hydrogen production from crude glycerol

Cristiano Varrone, Silvia Rosa, Floriana Fiocchetti, Barbara Giussani, Giulio Izzo, Giulia Massini, Antonella Marone, Antonella Signorini, Aijie Wang

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Enriched activated sludge that can effectively convert crude glycerol into bio-hydrogen was selected by an eco-biotechnological approach, in very strict conditions, using biodiesel-derived glycerol as the only carbon source. The thus obtained functional consortium was characterized by the genera Klebsiella, Escherichia/Shigella and Cupriavidus. During enrichment, the dominant metabolic end-product shifted from a 1,3 propanediol to ethanol, with a concomitant increase of the hydrogen yield from 0.18 ± 0.003 to 0.66 ± 0.06 mol/mol and an almost five-fold increase of the hydrogen production. Glycerol degradation efficiency showed an increase of around 50%. In optimized and upscaled conditions it was possible to obtain a hydrogen production rate of 2960 mL H2/L/day ± 185 at a near stoichiometric yield (of 0.90 mol/mol ± 0.01), with a carbon recovery of almost 90%, both in sterile and non-sterile conditions. Glycerol was almost totally degraded (degradation efficiency of 97.42% ± 0.98), independently of the glycerol type used. Copyright © 2012, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)1319 - 1331
Number of pages13
JournalInternational Journal of Hydrogen Energy
Volume38
Issue number3
DOIs
Publication statusPublished - 6 Feb 2013

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

Cite this