Eumelanin Coated PLA Electrospun Micro Fibers as Bioinspired Cradle for SH-SY5Y Neuroblastoma Cells Growth and Maturation

Ines Fasolino, Irene Bonadies, Luigi Ambrosio, Maria Grazia Raucci, Cosimo Carfagna, Federica Maria Caso, Francesca Cimino, Alessandro Pezzella

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Abstract

Within the framework of neurodegenerative disorder therapies, the fabrication of 3D eumelanin architectures represents a novel strategy to realize tissue-engineering scaffolds for neuronal cell growth and control by providing both mechanical support and biological signals. Here, an appropriate procedure combining electrospinning, spin coating and solid-state polymerization process is established to realize the scaffolds. For biological analysis, a human derived cell line SH-SY5Y from neuroblastoma is used. Cell maturation on eumelanin microfibers, random and aligned, is evaluated by using confocal analysis and specific markers of differentiating neurons (βIII tubulin and GAP-43 expression). Cell morphology is tested by SEM analysis and immunofluorescence techniques. As results, eumelanin coated microfibers prove capable to support biological response in terms of cell survival, adhesion and spreading and to promote cell differentiation toward a more mature neuronal phenotype as confirmed by GAP-43 expression over the culture.
Original languageEnglish
Pages (from-to)40070 - 40076
Number of pages7
JournalACS Applied Materials and Interfaces
Volume9
Issue number46
DOIs
Publication statusPublished - 22 Nov 2017
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Materials Science(all)

Cite this

Fasolino, I., Bonadies, I., Ambrosio, L., Raucci, M. G., Carfagna, C., Caso, F. M., Cimino, F., & Pezzella, A. (2017). Eumelanin Coated PLA Electrospun Micro Fibers as Bioinspired Cradle for SH-SY5Y Neuroblastoma Cells Growth and Maturation. ACS Applied Materials and Interfaces, 9(46), 40070 - 40076. https://doi.org/10.1021/acsami.7b13257