Evaluation of genotoxic potential of waters from two Italian rivers in Gammarus elvirae (Amphipoda)

Lucilla Ronci, Valentina Iannilli, Elvira De Matthaeis, Giovanna Di Donato, Andrea Setini

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The aim of the present work is to evaluate the genotoxic impact of contaminants along the whole course of Ninfa-Sisto and Amaseno (Latium, Italy) rivers. The authors performed the alkaline Comet assay to assess DNA damage in the freshwater amphipod Gammarus elvirae, exposed ex situ for 24 hours and 7 days to water collected at different sites. The assay, applied on haemocytes, provides a sensitive tool to reveal effects even at low concentrations of pollutants. The results indicate significant increase of DNA damage along the course of the two rivers, compared to the unpolluted upstream sites, even if the analytes do not exceed the permissible limits. Moreover, the results show that there is not a linear correlation between the concentration of analytes and DNA damage. Based on this study's results, it would be desirable to use Comet assay, on proposed test species, as an early warning method to detect genotoxic potential of waters.
Original languageEnglish
Pages (from-to)2008 - 2017
Number of pages10
JournalWater Environment Research
Issue number11
Publication statusPublished - 1 Nov 2015
Externally publishedYes


All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Ecological Modelling
  • Water Science and Technology
  • Waste Management and Disposal
  • Pollution

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