Evaluation of genotoxicity of oral exposure to tetravalent vanadium in vivo

Paola Villani, Eugenia Cordelli, Paola Leopardi, Ester Siniscalchi, Enrico Veschetti, Anna Maria Fresegna, Riccardo Crebelli

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The trace element vanadium interacts with living cells, in which it exerts a variety of biological effects depending on its chemical form and oxidation state. Tetravalent vanadium was shown to affect several genotoxicity end-points in vitro, but its genotoxic potential in vivo is not elucidated. In this study, the genotoxic effects induced in vivo by subacute oral exposure to vanadyl sulphate (VOSO4), a tetravalent vanadium salt, were investigated. To this aim male CD1 mice were administered with VOSO4in drinking water over the dose range 2-1000 mg/l for 5 weeks. The incidence of micronucleated blood reticulocytes was measured along treatment period. At the end of treatment, micronuclei in both blood reticulocytes and bone marrow polychromatic erythocytes were determined; in addition, DNA lesions detectable by comet assay were assessed in marrow and testicular cells. Tissue distribution of vanadium at sacrifice was determined by atomic absorption spectrometry. Comet assays and the analysis of micronuclei in polychromatic erythrocytes did not reveal treatment related effects. A slight increase in micronucleated reticulocytes, with no relationship with the administered dose, was observed in some treated groups. The determination of vanadium content in kidney, liver, spleen, bone, stomach, small intestine and testis highlighted low internal exposure, especially in soft tissues. Overall, data indicate scarce bioavailability for orally administered tetravalent vanadium, and lack of significant genotoxic potential in vivo. © 2007 Elsevier Ireland Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)11 - 18
Number of pages8
JournalToxicology Letters
Issue number1
Publication statusPublished - 5 Apr 2007
Externally publishedYes


All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

Villani, P., Cordelli, E., Leopardi, P., Siniscalchi, E., Veschetti, E., Fresegna, A. M., & Crebelli, R. (2007). Evaluation of genotoxicity of oral exposure to tetravalent vanadium in vivo. Toxicology Letters, 170(1), 11 - 18. https://doi.org/10.1016/j.toxlet.2006.07.343