First evidence on the occurrence and dynamics of Dehalococcoides mccartyi PCB-dechlorinase genes in marine sediment during Aroclor1254 reductive dechlorination

B. Matturro, M. Di Lenola, C. Ubaldi, S. Rossetti

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The present study evaluates the PCB-dehalorespiring capabilities and dynamics of indigenous Dehalococcoides mccartyi population in a PCB contaminated marine sediment. Specialized PCB-dechlorinase genes pcbA1, pcbA4 and pcbA5 previously characterized in pure cultures of D. mccartyi, were here found for the first time in environmental samples. Reductive dechlorination was stimulated by spiking Aroclor1254 to the sediment and by imposing strictly anaerobic conditions both with and without bioaugmentation with a Dehalococcoides mccartyi enrichment culture. In line with the contaminant dechlorination kinetics, Dehalococcoides population increased during the entire incubation period showing growth yields of 4.94E + 07 Dehalococcoides per μmol Cl− 1and 7.30E + 05 Dehalococcoides per μmol Cl− 1in the marine sediment with and without bioaugmentation respectively. The pcbA4 and pcbA5 dechlorinase genes, and to a lesser extent pcbA1 gene, were enriched during the anaerobic incubation suggesting their role in Aroclor1254 dechlorination under salinity conditions.
Original languageEnglish
Pages (from-to)189 - 194
Number of pages6
JournalMarine Pollution Bulletin
Volume112
Issue number1-2
DOIs
Publication statusPublished - 15 Nov 2016
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Oceanography
  • Aquatic Science
  • Pollution

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