Flavonoid metabolism in Forsythia flowers

Carlo Rosati, Alain Cadic, Michel Duron, Marie-Josèphe Amiot, Max Tacchini, Stefan Martens, Gert Forkmann

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Abstract

The flavonoid pathway metabolism was studied in petals and sepals of Forsythia X intermedia cv. 'Spring Glory'. The activities of the flavonoid enzymes chalcone synthase (CHS), flavanone 3-hydroxylase (FHT) and flavonol synthase (FLS) were measured. The dihydroflavonol 4-reductase (DFR) role was also studied by comparing flavonoid accumulation in transgenic plants for a heterologous DFR gene and wild-type F. X intermedia cv. 'Spring Glory', already investigated for DFR gene expression and activity. HPLC analyses complemented enzymatic investigations, showing that: (i) rutin (quercetin 3- rutinoside) is the major flavonol accumulated in petals and sepals (ca. 90% of the flavonol pool) and; (ii) quercetin and cyanidin derivatives are the exclusive flavonols and anthocyanins in sepals, respectively. The overall data demonstrated that the flavonoid pathway in F. X intermedia flower organs leads to the major accumulation of 3',4'-dihydroxylated compounds, and that 3'-hydroxylation of the B-ring occurs mainly at flavonoid intermediate stage(s). Comparative HPLC analyses of F. X intermedia cv. 'Spring Glory' and three other genotypes (F. giraldiana, F. X intermedia 'Korfor' Goldzauber® and F. ovata 'Robusta') confirmed the major production of flavonols by Forsythia flavonoid metabolism and suggested a method for screening Forsythia genotypes based on anthocyanin accumulation pattern in sepals.
Original languageEnglish
Pages (from-to)133 - 140
Number of pages8
JournalPlant Science
Volume139
Issue number2
DOIs
Publication statusPublished - 31 Dec 1998
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Rosati, C., Cadic, A., Duron, M., Amiot, M-J., Tacchini, M., Martens, S., & Forkmann, G. (1998). Flavonoid metabolism in Forsythia flowers. Plant Science, 139(2), 133 - 140. https://doi.org/10.1016/S0168-9452(98)00180-0