Flow cytometric and immunohistochemical correlations in high incidence human solid tumors

Donatella Tirindelli Danesi, Marcello Spanò, Fabiana Antonini, Pierluigi Altavista, Piera Catalano, Eugenia Cordelli, Patrizio Pasqualetti, Criselda Santacroce, Maria Gabriella Toscano, Antonella Mecozzi, Alfredo Fabiano

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Abstract

475 patients with carcinoma at different sites (141 colon-rectum; 102 breast; 50 stomach; 48 kidney; 46 head and neck; 41 bladder; 47 other sites) submitted to surgery have been analyzed after histopathological staging and grading, by flow cytometry (monoparametric DNA content analysis) and immunohistochemistry (p53, c-erbB-2, and PCNA expression). In breast cancer patients the presence of receptors for estrogen (ER) and progesterone (PGR) has also been determined. Flow cytometry-derived parameters were DNA ploidy, fraction of cells in S-phase (SPF), and DNA content heterogeneity (multiclonal stem cell lines with different DNA index and/or more than one subpopulations with different ploidy levels in different samples from the same tumor). Correlations of the results obtained by the different techniques have been attempted by the non-parametric Spearman's rank correlation approach. Significant associations (P < 0.05) were found between the histopathological, immunohistochemical and flow cytometric parameters considered in some anatomical regions, such as stomach (p53 vs DNA content aneuploidy and vs heterogeneity), colon-rectum (TNM vs p53 and vs heterogeneity), bladder (grading vs DNA content aneuploidy and vs heterogeneity). Tumor heterogeneity proved to be dependent on the number of tumor samples taken. The results of this preliminary assessment will subsequently be compared with the data obtained from a currently ongoing follow-up survey.
Original languageEnglish
Pages (from-to)689 - 697
Number of pages9
JournalTumori
Volume83
Issue number3
Publication statusPublished - May 1997
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Cancer Research

Cite this

Danesi, D. T., Spanò, M., Antonini, F., Altavista, P., Catalano, P., Cordelli, E., ... Fabiano, A. (1997). Flow cytometric and immunohistochemical correlations in high incidence human solid tumors. Tumori, 83(3), 689 - 697.