Frequency spectrum of induced transmembrane potential and permeabilization efficacy of bipolar electric pulses

Caterina Merla, Andrei G. Pakhomov, Iurii Semenov, P. Thomas Vernier

Research output: Contribution to journalArticle

14 Citations (Scopus)


In this paper a simple prediction method for the bipolar pulse cancellation effect is proposed, based on the frequency analysis of the TMP spectra of a single cell and the computed relative global spectral content up to a defined frequency threshold. We present a spectral analysis of pulses applied in experiments, and we extract the induced TMP from a microdosimetric model of the cell. The induced TMP computation is carried out on a hemispherical multi-layered cell model in the time domain. The analysis is presented for a variety of unipolar and bipolar input signals in the nanosecond and the microsecond time scales. Our evaluations are in good agreement with experimental results for bipolar pulse cancellation of electropermeabilization-induced Ca2+influx using 300 ns, 750 kV/m pulses and with other results reported in recent literature.
Original languageEnglish
Pages (from-to)1282 - 1290
Number of pages9
JournalBiochimica et Biophysica Acta - Biomembranes
Issue number7
Publication statusPublished - 1 Jul 2017
Externally publishedYes


All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Cell Biology

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