In this paper a simple prediction method for the bipolar pulse cancellation effect is proposed, based on the frequency analysis of the TMP spectra of a single cell and the computed relative global spectral content up to a defined frequency threshold. We present a spectral analysis of pulses applied in experiments, and we extract the induced TMP from a microdosimetric model of the cell. The induced TMP computation is carried out on a hemispherical multi-layered cell model in the time domain. The analysis is presented for a variety of unipolar and bipolar input signals in the nanosecond and the microsecond time scales. Our evaluations are in good agreement with experimental results for bipolar pulse cancellation of electropermeabilization-induced Ca2+influx using 300 ns, 750 kV/m pulses and with other results reported in recent literature.
All Science Journal Classification (ASJC) codes
- Cell Biology
Merla, C., Pakhomov, A. G., Semenov, I., & Vernier, P. T. (2017). Frequency spectrum of induced transmembrane potential and permeabilization efficacy of bipolar electric pulses. Biochimica et Biophysica Acta - Biomembranes, 1859(7), 1282 - 1290. https://doi.org/10.1016/j.bbamem.2017.04.014