FT-IR imaging spectroscopy as a complementary analytical technique to monitor lipids as biomarkers to high-LET (linear energy transfer) radiation

Mariangela Cestelli Guidi, Chiara Mirri, Emiliano Fratini, Valerio Licursi, Augusto Marcelli

Research output: Contribution to journalArticle

Abstract

Skin reaction to irradiation is a key factor to understand the role of high-linear energy transfer radiation in the treatment of cancer, as well as the tolerance of the damage and the functional changes induced in the contiguous normal tissues. Moreover, low doses of neutron are a potentially dangerous hazard during low earth astronautic orbit missions and aircrew activities. To this purpose, mice skin irradiated with 14 Mev neutrons was studied in term of its genomic response combining cDNA microarrays methodology, to analyze transcriptomic changes, and high resolution Fourier transform-infra red (FT-IR) spectroscopy, to monitor the lipid content and distribution in dorsal skin sections after the neutron irradiation treatment. FT-IR spectroscopy imaging provides both spatial and biochemical information on a micrometric scale without being destructive, thus representing a novel and powerful approach in the context of revealing radiation exposure effects. © Accademia Nazionale dei Lincei 2014.
Original languageEnglish
Pages (from-to)-
JournalATTI Della Accademia Nazionale Dei Lincei Rendiconti Lincei Scienze Fisiche E Naturali
Volume25
Issue numberSUPPL. 1
DOIs
Publication statusPublished - 2014
Externally publishedYes

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Environmental Science(all)
  • Agricultural and Biological Sciences(all)
  • Earth and Planetary Sciences(all)

Cite this