Gadolinium in human glioblastoma cells for gadolinium neutron capture therapy

G. De Stasio, P. Casalbore, R. Pallini, B. Gilbert, F. Sanità, M.T. Ciotti, G. Rosi, A. Festinesi, L.M. Larocca, A. Rinelli, D. Perret, D.W. Mogk, P. Perfetti, M.P. Mehta, D. Mercanti

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Abstract

157Gd is a potential agent for neutron capture cancer therapy (GdNCT). We directly observed the microdistribution of Gd in cultured human glioblastoma cells exposed to Gd-diethylenetriaminepentaacetic acid (Gd-DTPA). We demonstrated, with three independent techniques, that Gd-DTPA penetrates the plasma membrane, and we observed no deleterious effect on cell survival. A systematic microchemical analysis revealed a higher Gd accumulation in cell nuclei compared with cytoplasm. This is significant for prospective GdNCT because the proximity of Gd to DNA increases the cell-killing potential of the short-range, high-energy electrons emitted during the neutron capture reaction. We also exposed Gd-containing cells to thermal neutrons and demonstrated the GdNC reaction effectiveness in inducing cell death. These results in vitro stimulated in vivo Gd-DTPA uptake studies, currently underway, in human glioblastoma patients.
Original languageEnglish
Pages (from-to)4272 - 4277
Number of pages6
JournalCancer Research
Volume61
Issue number10
Publication statusPublished - 15 May 2001
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

De Stasio, G., Casalbore, P., Pallini, R., Gilbert, B., Sanità, F., Ciotti, M. T., ... Mercanti, D. (2001). Gadolinium in human glioblastoma cells for gadolinium neutron capture therapy. Cancer Research, 61(10), 4272 - 4277.