Heat-shock protein 70 from plant biofactories of recombinant antigens activate multiepitope-targeted immune responses

Giampaolo Buriani, Camillo Mancini, Eugenio Benvenuto, Selene Baschieri

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9 Citations (Scopus)

Abstract

Although a physiological role of heat-shock proteins (HSP) in antigen presentation and immune response activation has not been directly demonstrated, their use as vaccine components is under clinical trial. We have previously demonstrated that the structure of plant-derived HSP70 (pHSP70) can be superimposed to the mammalian homologue and similarly to the mammalian counterpart, pHSP70-polypeptide complexes can activate the immune system. It is here shown that pHSP70 purified from plant tissues transiently expressing the influenza virus nucleoprotein are able to induce both the activation of major histocompatibility complex class I-restricted polyclonal T-cell responses and antibody production in mice of different haplotypes without the need of adjuvant co-delivery. These results indicate that pHSP70 derived from plants producing recombinant antigens may be used to formulate multiepitope vaccines. © 2012 The Authors. Plant Biotechnology Journal © 2012 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd.
Original languageEnglish
Pages (from-to)363 - 371
Number of pages9
JournalPlant Biotechnology Journal
Volume10
Issue number3
DOIs
Publication statusPublished - Apr 2012
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

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