An efficient protocol was developed for rapid clonal propagation of the important medicinal plant, Centella asiatica (L.) Urban, using shoot tip culture. High frequency bud break (88 %) and multiple shoot formation (16.8 shoots/shoot tip) were induced from a shoot tip segment, which was cultured on MS medium supplemented with 6-benzylaminopurine (BAP) (17.76 μM) and gibberillic acid (GA3) (1.44 μM). Half-strength Murashige and Skoog (MS) medium supplemented with naphthalen acetic acid (NAA) (10.74 μM) induced the maximum (27.66) number of roots. Plantlets with 3-4 fully expanded leaves and well-developed roots were successfully transferred to potted soil which exhibited a 95% survival. The protocol enables the harvest of more than 25,000 plantlets within 160 days starting from a single shoot tip explant. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
All Science Journal Classification (ASJC) codes
- Environmental Engineering
Sivakumar, G., Alagumanian, S., & Rao, M. V. (2006). High frequency in vitro multiplication of Centella asiatica: An important industrial medicinal herb. Engineering in Life Sciences, 6(6), 597 - 601. https://doi.org/10.1002/elsc.200620159