High-sensitive impedimetric aptasensor for detection ochratoxin A in food

Gabriela Castillo, Ilaria Lamberti, Lucia Mosiello, Tibor Hianik

Research output: Contribution to conferencePaper

2 Citations (Scopus)

Abstract

We report a high sensitive biosensor based on DNA aptamers for detection ochratoxin A (OTA). The thiolated DNA aptamers specific to OTA have been immobilised to a surface of gold electrode. The electrochemical impedance spectroscopy (EIS) at presence of redox probe [Fe(CN)6]-3/-4has been used for determination of charge transfer resistance, Rct, following addition of OTA. We have shown that Rctincreased with increasing OTA in a range of 0.1-100 nM. The sensor revealed high sensitivity (the limit of detection was 0.44 nM), selectivity and was regenerable. The sensor was validated in coffee extract. © 2012 Springer Science+Business Media, LLC.
Original languageEnglish
DOIs
Publication statusPublished - 2012
Externally publishedYes
Event16th Conference on Italian Association of Sensors and Microsystems, AISEM 2011 - , Italy
Duration: 1 Jan 2012 → …

Conference

Conference16th Conference on Italian Association of Sensors and Microsystems, AISEM 2011
CountryItaly
Period1/1/12 → …

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All Science Journal Classification (ASJC) codes

  • Industrial and Manufacturing Engineering

Cite this

Castillo, G., Lamberti, I., Mosiello, L., & Hianik, T. (2012). High-sensitive impedimetric aptasensor for detection ochratoxin A in food. Paper presented at 16th Conference on Italian Association of Sensors and Microsystems, AISEM 2011, Italy. https://doi.org/10.1007/978-1-4614-0935-9_6