HPLC screening of natural vitamin E from mediterranean plant biofactories - A basic tool for pilot-scale bioreactors production of α-tocopherol

G. Sivakumar, L. Bacchetta, R. Gatti, G. Zappa

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15 Citations (Scopus)


The study was performed in order to investigate a simple, efficient, reliable and rapid method of extracting and quantifying natural vitamin E for pressurized liquid extraction (PLE) as well as high-performance liquid chromatography (HPLC) analysis. Lyophilized Corylus avellana L. nut samples were powdered by high-speed milling with Waring blender for 40 s. α-Tocopherol was extracted from the nut tissue powder using dehydrated hexane fortified with 0.01% butylated hydroxytoluene (BHT, co-antioxidant). The rate of α-tocopherol accumulation showed differences among nut samples collected in different areas of Italy. Sarda Piccola nut biofactory contained higher amount (81.17 μg/g d.w) of α-tocopherol than other-local eleven Italian cultivar nuts. These results provide insight into the biofactory basis for α-tocopherol accumulation in hazelnut and give the suitable cultivar tissues to establish pilot-scale bioreactors production of natural bioactive vitamin E. © 2005 Elsevier GmbH. All rights reserved.
Original languageEnglish
Pages (from-to)1280 - 1283
Number of pages4
JournalJournal of Plant Physiology
Issue number11
Publication statusPublished - 15 Nov 2005
Externally publishedYes


All Science Journal Classification (ASJC) codes

  • Plant Science

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