Treatment of leukemia cells with 1, 25-dihydroxyvitamin D3may overcome their differentiation block and lead to the transition from myeloblasts to monocytes. To identify microRNA-mRNA networks relevant for myeloid differentiation, we profiled the expression of mRNAs and microRNAs associated to the low- and high-density ribosomal fractions in leukemic cells and in their differentiated monocytic counterpart. Intersection between mRNAs shifted across the fractions after treatment with putative target genes of modulated microRNAs showed a series of molecular networks relevant for the monocyte cell fate determination, as for example the post-transcriptional regulation of the Polo-like kinase 1 (PLK1) by miR-22–3p and let-7e-5p.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology
Fontemaggi, G., Bellissimo, T., Donzelli, S., Iosue, I., Benassi, B., Bellotti, G., Blandino, G., & Fazi, F. (2015). Identification of post-transcriptional regulatory networks during myeloblast-to-monocyte differentiation transition. RNA Biology, 12(7), 690 - 700. https://doi.org/10.1080/15476286.2015.1044194