This study reports the immunomodulatory activity on human Monocyte Derived Dendritic Cells (MDDCs) of a vaccine preparation shown to be effective against an HPV16-related tumour in an animal model. The vaccine is composed of extract from Nicotiana benthamiana leaves containing HPV16 E7 protein expressed by a potato virus X-derived vector (NbPVX-E7). The effect of the extract was evaluated on MDDC differentiation and maturation by monitoring the phenotypic expression of specific markers. The results show that NbPVX-E7 does not induce monocyte differentiation to dendritic cells, but does induce MDDC maturation. Plant extract does not influence MDDC-uptake of E7-FITC while it significantly improves the Ovalbumin-FITC uptake, considered as a model antigen. Importantly, NbPVX-E7-pulsed MDDCs/PBMCs are able to prime human blood-derived lymphocytes from healthy individuals to induce HPV16 E7-specific cytotoxic activity. This is a propaedeutic study for a possible use of E7-containing plant extract in human immunotherapy of HPV-related lesions. Copyright © by Biolife, s.a.s.
|Pages (from-to)||967 - 978|
|Number of pages||12|
|Journal||International Journal of Immunopathology and Pharmacology|
|Publication status||Published - 2009|
All Science Journal Classification (ASJC) codes
- Immunology and Allergy
Di Bonito, P., Grasso, F., Mangino, G., Massa, S., Illiano, E., Franconi, R., ... Giorgi, C. (2009). Immunomodulatory activity of a plant extract containing human papillomavirus 16-E7 protein in human monocyte-derived dendritic cells. International Journal of Immunopathology and Pharmacology, 22(4), 967 - 978. https://doi.org/10.1177/039463200902200412