In vitro adventitious shoot formation on cotyledons of Pinus pinea

María Victoria González, Manuel Rey, Raffaela Tavazza, Stefano La Malfa, Luigi Cuozzo, Giorgio Ancora

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Plant regeneration was obtained from adventitious buds induced in isolated cotyledons of Italian stone pine (Pinus pinea L.). The best results for bud induction were obtained by using half-strength LePoivre medium with 4.5 μM 6-benzyladenine for 30 days. Shoot elongation was achieved in the same medium without growth regulators but with the addition of 0.5% activated charcoal. The induction medium was the best also for shoot multiplication, but it was necessary to include subcultures on elongation medium. The slow elongation rate of adventitious shoots remains the greatest obstacle to multiplication. Root formation (15%) after 5 months was observed when shoots were cultured on elongation medium for long periods.
Original languageEnglish
Pages (from-to)749 - 750
Number of pages2
JournalHortscience: A Publication of the American Society for Hortcultural Science
Issue number4
Publication statusPublished - 1998
Externally publishedYes


All Science Journal Classification (ASJC) codes

  • Horticulture

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