Inhibition of Proliferation by c-myb Antisense RNA and Oligodeoxynucleotides in Transformed Neuroectodermal Cell Lines

Giuseppe Raschella, Anna Negroni, Sabina Pucci, Antonino Romeo

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Abstract

Transfection of a neuroblastoma cell line with expression vector scontaining two different segments of human c-mybcomplementary DNAin antisense orientation yielded far fewer transfectant clones than didthe transaction with the identical segments in sense orientation. In cellclones expressing c-myb antisense RNA, levels of the c-myb proteinwere down-regulated and the proliferation rate was slower than that of cells transfected with sense constructs or the untransfected parental cellline. Treatment of neuroblastoma and neuroepithelioma cell lines with ac-myb antisense oligodeoxynucleotide strongly inhibited cell growth. These data indicate a definite involvement of c-myh in the proliferationof neuroectodermal tumor cells extending the role of this protooncogenebeyond the hematopoietic system. The availability of cell clones thattranscribe c-myh antisense RNA provides a useful tool to study theinvolvement of other genes in the proliferation and differentiation ofneuroblastoma cells. © 1992, American Association for Cancer Research. All rights reserved.
Original languageEnglish
Pages (from-to)4221 - 4226
Number of pages6
JournalCancer Research
Volume52
Issue number15
Publication statusPublished - 1992
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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