Interphase cytogenetics and flow cytometry analyses of renal tumours

M.G. Grollino, D. Cavallo, F. Di Silverio, M. Rocchi, R. De Vita

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6 Citations (Scopus)

Abstract

Fluorescent in situ hybridization (FISH) can be used to determine chromosome changes in human neoplasia. In our study we have tested the feasibility of FISH to interphase cells of renal carcinoma to evaluate chromosome aneuploidies. We carried out in parallel in situ hybridization and flow cytometric studies in order to evaluate the possible correlation between numerical chromosome abnormalities and ploidies detected by flow cytometry (FCM). The ploidy of chromosomes 7, 11, 17 and 18 was investigated in three cases of this tumour utilizing specific probes. We found evaluable and comparable results in every case of renal carcinoma analyzed for both FISH and DNA FCM analyses and our results indicate that fluorescent in situ hybridization with chromosome-specific repetitive DNA probes can serve as a cytogenetic tool for the detection of numerical specific chromosome abnormalities of interphase nuclei of renal carcinoma.
Original languageEnglish
Pages (from-to)2239 - 2244
Number of pages6
JournalAnticancer Research
Volume13
Issue number6 A
Publication statusPublished - 1993
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Grollino, M. G., Cavallo, D., Di Silverio, F., Rocchi, M., & De Vita, R. (1993). Interphase cytogenetics and flow cytometry analyses of renal tumours. Anticancer Research, 13(6 A), 2239 - 2244.