Investigation on metabolic fingerprint diversity of different Agrobacterium strains and cereal transformation

A. Latini, F. Tasso, M. Sperandei, F. Morelli, M. Pugnali, M. Dettori, C. Cantale, P. Galeffi

Research output: Contribution to journalArticle

Abstract

Many factors affect the Agrobacterium-mediated transformation of monocotyledons as plant genotype, Agrobacterium strain and binary vectors. In order to find the optimal conditions for accomplishing the cereal transformation by Agrobacterium we compared the metabolic fingerprinting of different bacterial strains, obtained by the Biolog system, in view to highlight potential correlation between their metabolic diversity and their transformation abilities. Two Agrobacterium strains, representative for the octopine and nopaline groups, have been tested to compare their transformation capability in two dicotyledonous species and in cereals. In addition, the regenerative abilities of various Triticum aestivum L. and Triticale varieties were also tested with the final aim of finding the better conditions for the generation of the wheat transgenic plants.
Original languageEnglish
Pages (from-to)143 - 150
Number of pages8
JournalJournal of Genetics and Breeding
Volume61
Issue number1-2
Publication statusPublished - Jan 2007
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science
  • Horticulture

Cite this

Latini, A., Tasso, F., Sperandei, M., Morelli, F., Pugnali, M., Dettori, M., ... Galeffi, P. (2007). Investigation on metabolic fingerprint diversity of different Agrobacterium strains and cereal transformation. Journal of Genetics and Breeding, 61(1-2), 143 - 150.