Label-free DNA analysis system based On lab-on-glass technology

D. Caputo, M. Ceccarelli, G. De Cesare, R. Intrieri, A. Nascetti, R. Scipinotti, P. Martufi, M. Tucci

Research output: Contribution to conferencePaper

Abstract

In this paper we present a Lab-On-Glass system for label free DNA analysis that integrates a polymerase chain reaction (PCR) chamber for DNA amplification, an electrowetting-based droplet-handling system and a hydrogenated amorphous silicon ultraviolet sensor array for DNA detection. The photosensors allow to measure the absorbance of the biomolecules at 254 nm to reveal and quantify the DNA sample. The system is fabricated using thinfilm and thick-film technologies on a conventional microscope glass slide and its design has been carried out by taking into account the application requirements as well as the compatibility of the process steps. The PCR chamber incorporates a thin metal film heater optimized for uniform temperature distribution and an amorphous silicon diode for temperature measurement. The diode shows a sensitivity of -3.3mV/K in the range 30-80 °C when biased with a forward current of 10nA. A successful droplet handling capability was achieved using a hydrophobic Teflon layer deposited oer the silicon nitride film used to passivate both the heater and the ultraviolet sensor array. The voltages needed to successfully move a 2μ⌉ water droplet were in the range of 80 Volts. Finally, we demonstrated the sensor ability to distinguish between single- and double-tranded molecules functionalized on a quartz substrate. © 2008 IEEE.
Original languageEnglish
DOIs
Publication statusPublished - 2008
Externally publishedYes
Event2008 IEEE Sensors, SENSORS 2008 - , Italy
Duration: 1 Jan 2008 → …

Conference

Conference2008 IEEE Sensors, SENSORS 2008
CountryItaly
Period1/1/08 → …

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All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

Cite this

Caputo, D., Ceccarelli, M., De Cesare, G., Intrieri, R., Nascetti, A., Scipinotti, R., ... Tucci, M. (2008). Label-free DNA analysis system based On lab-on-glass technology. Paper presented at 2008 IEEE Sensors, SENSORS 2008, Italy. https://doi.org/10.1109/ICSENS.2008.4716500