Lack of Correlation between N-myc and MAX Expression in Neuroblastoma Tumors and in Cell Lines: Implication for N-iwyc-MAX Complex Formation

Giuseppe Raschella, Antonino Romeo, Anna Negroni, Sabina Pucci, Carlo Dominici, Manuel A. Castello, Paola Bevilacqua, Armando Felsani

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Abstract

Detectable levels of MAX messenger RNA were found in a set of human neuroblastoma tumors and established cell lines. MAX mRNA levels were independent of tumor stage and N-myc genomic amplification. By contrast, N-myc mRNA transcripts were detectable only in tumors with amplification of N-myc gene and in cell lines. Analysis by reverse transcriptase polymerase chain reaction and hybridization to specific oligode-oxynucleotide probes revealed approximately equal amounts of two MAX transcripts in all cases analyzed. ImmunoprecipiUtions with a specific antibody to MAX detected two proteins of Mr 21,000 and 22,000 in approximately equal amounts in all neuroblastoma lines regardless of N-mvc amplification and/or expression. On the other hand, protein binding to the myc DNA consensus sequence correlated with N-myc expression in neuroblastoma cells. Thus, N-myc expression might be a limiting factor in the formation of the N-myc-MAX heterodimer in neuroblastomas. © 1994, American Association for Cancer Research. All rights reserved.
Original languageEnglish
Pages (from-to)2251 - 2255
Number of pages5
JournalCancer Research
Volume54
Issue number8
Publication statusPublished - 1994
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Raschella, G., Romeo, A., Negroni, A., Pucci, S., Dominici, C., Castello, M. A., ... Felsani, A. (1994). Lack of Correlation between N-myc and MAX Expression in Neuroblastoma Tumors and in Cell Lines: Implication for N-iwyc-MAX Complex Formation. Cancer Research, 54(8), 2251 - 2255.