Laser-assisted fabrication of biomolecular sensing microarrays

Serena Gagliardi, Bruno Rapone, Lucia Mosiello, Domenico Luciani, Annamaria Gerardino, Piero Morales

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

With the aim of obtaining high density biosensing arrays we use pulsed laser deposition to immobilize functional biomolecules on useful surfaces, and micro- and nanopatterning techniques for fabrication of prototype immunosensing bioarrays. We report biological activity tests demonstrating the functional properties of the immobilized proteins and atomic force microscopy characterization of films of nanometric dimensions. Laser-fabricated immunofluorescent arrays are analyzed to check that the intensity and contrast of the sensing sites allow efficient device fabrication. We have also developed an elementary array of heterogeneous reaction sites and tested its performance by simultaneous incubation with the different specific antigens. © 2007 IEEE.
Original languageEnglish
Pages (from-to)242 - 248
Number of pages7
JournalIEEE Transactions on Nanobioscience
Volume6
Issue number3
DOIs
Publication statusPublished - Sep 2007
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Pharmaceutical Science
  • Computer Science Applications
  • Electrical and Electronic Engineering

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