Light scattering measurement of nanoparticle aggregates by Scanning Flow Cytometer

R. Fantoni, L. Fiorani, A. Palucci, K.A. Semyanov, V. Spizzichino

Research output: Contribution to journalArticle


Light scattering of nanoparticle aggregates has been measured by a Laser Scanning Flow Cytometer (LSFC) equipped with two channels: one for particles Side scattering (SSC) detection and the other for measurements of the light scattered within an azimuth solid angle in the range 10°-70° (Light Scattering Pattern, LSP). By studying in particular SiC nanostructures we demonstrate the ability of the developed LSFC apparatus to dimensionally characterize and classify non spherical submicron particles. This is possible thanks to its unique property to analyze with a high rate singular particles in suspension and to retrieve their scattering pattern in a wide angular range. Measurements of the SSC and LSP of polystyrene microspheres have been performed to obtain empirical relationships between the LSP integral and particle size. Simulations on nanoparticle aggregate models have been carried out by the Discrete Dipole Approximation (DDA) method. The so obtained forward light scattering pattern for modeled structures has been compared to the experimental results. Moreover, DLS (Dynamic Light Scattering) measurements on the same samples have been performed to compare their data on size distribution to ones deriving from cytometer measurements. The comparison between DLS results and those obtained by the LSFC highlights advantages and limits of the two techniques versus the specific type of particles here studied.
Original languageEnglish
Pages (from-to)2474 - 2481
Number of pages8
JournalJournal of Optoelectronics and Advanced Materials
Issue number9
Publication statusPublished - Sep 2008
Externally publishedYes


All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this

Fantoni, R., Fiorani, L., Palucci, A., Semyanov, K. A., & Spizzichino, V. (2008). Light scattering measurement of nanoparticle aggregates by Scanning Flow Cytometer. Journal of Optoelectronics and Advanced Materials, 10(9), 2474 - 2481.