Car-R (carcinogenesis-resistant) and Car-S (carcinogenesis-susceptible) outbred mice, obtained by phenotypic selection from an initial intercross of eight inbred strains, show a >100fold difference in their susceptibility to two-stage skin tumorigenesis. We found that the lines carry a high degree of genetic polymorphism, with an average heterozygosity of 0.39. This polymorphism allowed the use of linkage disequilibrium (LD) and haplotype analysis for the mapping of a skin cancer modifier locus on Chr 7, in a short region of 6 cM, around the Tyr gene. Car-S mice inherited the susceptibility allele at this locus from the A/J, BALB/c, SJL/J, and SWR/J strains. Our results demonstrate the feasibility and usefulness of mapping disease genes by LD in phenotypically selected, genetically heterogeneous animals.
All Science Journal Classification (ASJC) codes
Peissel, B., Zaffaroni, D., Zanesi, N., Zedda, I., Manenti, G., Rebessi, S., ... Saran, A. (2000). Linkage disequilibrium and haplotype mapping of a skin cancer susceptibility locus in outbred mice. Mammalian Genome, 11(11), 979 - 981. https://doi.org/10.1007/s003350010184