Micronuclei in cytokinesis-blocked lymphocytes may predict patient response to radiotherapy

C. Catena, D. Conti, P. Parasacchi, P. Marenco, B. Bortolato, M. Botturi, M. Leoni, M. Portaluri, P.G. Paleani-Vettori, E. Righi

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Abstract

We have applied the cytokinesis-block micronucleus assay to peripheral blood lymphocytes of patients undergoing radiotherapy in pelvic and pulmonary sites, in order to evaluate the individual cytogenetic response. Our cytogenetic data correlated with the equivalent whole-body dose are homogeneous and compare well with the data presented by other authors. We have used an exponential mathematical formula to calculate the attenuation of the cytogenetic effect with time. The k coefficient (cytogenetic recovery factor) in the formula expresses the degree of attenuation. In lymyhocytes from patients after radiotherapy, the trend of the micronucleus frequency observed after 2 Gy of in vitro X-irradiation demonstrates that the cytogenetic effect obtained in vitro is added to that obtained in vivo. The k coefficient is inversely proportional to the micronucleus frequency observed after 2 Gy in vitro. The micronucleus assay and the cytogenetic recovery factor are proposed as suitable diagnostic tools for application in the field of radiotherapy.
Original languageEnglish
Pages (from-to)301 - 308
Number of pages8
JournalInternational Journal of Radiation Biology
Volume70
Issue number3
DOIs
Publication statusPublished - 1996
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging

Cite this

Catena, C., Conti, D., Parasacchi, P., Marenco, P., Bortolato, B., Botturi, M., ... Righi, E. (1996). Micronuclei in cytokinesis-blocked lymphocytes may predict patient response to radiotherapy. International Journal of Radiation Biology, 70(3), 301 - 308. https://doi.org/10.1080/095530096145030